Workshops

Workshop 1: Field Ionization, Field Desorption and Liquid Injection Field Desorption / Ionization
Organization: Mathias H. Linden, H. Bernhard Linden, Jürgen Gross
Part 1: 14:00 h – 15:15 h
Coffee Break
Part 2: 15:30 h – 16:45 h

Field ionization (FI), field desorption (FD), and liquid injection field desorption/ionization (LIFDI) are particularly soft ionization methods that can be applied to a wide variety of compound classes. The suitable variant of this family of ionization methods is chosen based on molecular weight, volatility, and polarity of the analyte. LIFDI is especially useful as it combines sample introduction under exclusion of moisture and air, reproducible sample deposition on the emitter with quick operation, and thus, short measurement times. Meanwhile, LIFDI sources can be obtained for a range of instruments including modern TOF and Orbitrap analyzers.

This workshop will fully explain the fundamentals of the techniques and demonstrate their capabilities along a range of elaborate examples. It will include instrumental topics such as mass analyzers to combine with and hyphenation of these soft ionization methods with various separation techniques.

Workshop 2: Mass Spectrometry imaging: Multimodal Approaches
Organization: Andreas Römpp, Bernhard Spengler
Part 1: 14:00 h – 15:15 h
Coffee Break
Part 2: 15:30 h – 16:45 h

Multimodal approaches are becoming increasingly popular in mass spectrometry imaging studies, ranging from the combination of different ionization types to the integration of complementary analytical techniques such as spectroscopy methods. Different application scenarios will be covered in this workshop along with practical considerations on experimental details and data handling.

Workshop 3: PTB-Workshop: Enabling Comparable Measurement Results through the Implementation of a Metrological Infrastructure
Organization: Claudia Swart, Gavin O’Connor
Part 1: 14:00 h – 15:15 h
Coffee Break
Part 2: 15:30 h – 16:45 h

The concept of “open data” becomes more and more important in all fields of science. Many funding organisations, such as DFG and the EU, requires open access to the data generated in successfully funded projects. However, open data are only useful when they are comparable and reliable. For over two decades the National Metrology Institutes (NMIs) have been working together to assess the worldwide comparability of measurement results. In order to achieve this, a constant assessment of the measurement results traceability and uncertainty were required. This workshop will address and explain these concepts and how mass spectrometry has an essential role in providing reference measurement. Although the process is slow, the agreement between NMIs in priority areas important to trade, is now well established. However, much has still to be achieved in the dissemination of traceability and the broader use of good analytical practices. The application of metrological concept of traceability guarantees that measurement results are comparable and, thus, reusable.

Part 1: a) Basic concepts in metrology, b) An introduction to the worldwide metrology infrastructure, c) How do NMIs achieve traceability of their results?, d) A general introduction to isotope dilution methods

Part 2: a) The development of IDMS reference measurement procedures for trace elements, organic molecules and simple proteins, b) Remaining challenges

Workshop 4 (Tutorial): Introduction to liquid chromatography for mass spectrometry
Organization: Waldemar Hoffmann, Michael Hoffmann und Martin Penkert
14:00 h – 15:15 h

Liquid chromatography coupled to mass spectrometry (LC-MS) is a powerful technique for the analysis of complex samples. Since its introduction in the 1980s LC-MS has undergone a rapid development and opened up various fields of analytical chemistry. Within this short course we will give a brief introduction into principles of chromatography, parameters influencing chromatographic separation and the challenges finding MS compatible chromatographic conditions. We will provide an overview over the most important separation techniques and explain its application using practical examples.

Workshop 5: ToF-SIMS and LEIS: Atomic and Molecular Characterisation of Surfaces and Interfaces
Organization: Birgit Hagenhoff
15:30 h – 16:45 h

This talk will present principles and applications of the surface analytical techniques LEIS (Low Energy Ion Scattering) and ToF-SIMS (Time-of-Flight Secondary Ion Mass Spectrometry).
Whereas LEIS allows a quantitative detection of elements being present in the outermost atomic layer, ToF-SIMS gives information on the elemental as well as molecular composition of the first 1-3 nm. By using additional sputtering, information down to some 10 µm can be gained.
For each technique the analytical principles will be discussed along with specific challenges. Applications will be presented in a second step including examples from areas like catalytic materials, polymers, glasses, coatings, OLEDs and biological materials.

Workshop 6: Protein Modification and Expression Analysis
Organization: Simone König
Part 1: 14:00 h – 15:15 h
Coffee Break
Part 2: 15:30 h – 16:45 h

The workshop is an introduction and overview into the field and directed at newcomers.
1. Simone König: Enzymatic digestion of proteins for mass spectrometry-based analysis
2. Christof Lenz: Data-independent acquisition mass spectrometry in omics research
3. Martin Scholz: Label-based and label-free strategies for protein quantification in photosynthetic organisms
4. Iris Finkemeier: Mass spectrometry-based profiling of protein lysine acetylation
5. Lolita Pierimoni / Christian Arlt: Cross-linking mass spectrometry
6. Simone König: Information content in shotgun / omics experiments

Workshop 7: Core Facilities
Organization: Christof Lenz
Date, time and place to be announced.

Workshop 8: Applications of Mass spectrometry in Pharmaceutical Industry
Organization: Jürgen Schäfer, Nico Zinn
Part 1: 14:00 h – 15:15 h
Coffee Break
Part 2: 15:30 h – 16:45 h

Mass spectrometry is a valuable tool in the pharmaceutical industry used at various stages of drug development. The aim of the workshop is to provide an overview of mass spectrometry focused techniques at the different stages of the pharmaceutical pipeline and its implications ranging from basic research to production. In addition, it should provide a forum for discussion and networking on pharma related Mass Spectrometry such as small molecule characterization, product impurities, proteomics and more.

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